Genotyping of Mycobacterium bovis isolates from badgers in four areas of the Republic of Ireland by restriction fragment length polymorphism analysis

作者: E. Costello , O. Flynn , F. Quigley , D. O'Grady , J. Griffin

DOI: 10.1136/VR.159.19.619

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摘要: An analysis of the molecular epidemiology Mycobacterium bovis in badgers was made four selected areas Republic Ireland which an intensive badger removal programme being carried out over a period five years. Tissue samples from 2310 were cultured. Restriction fragment length polymorphism (RFLP) with IS6110, polymorphic GC-rich sequence (PGRS) and direct repeat (DR) probes applied to isolates 398 badgers, 52 different rflp types identified. Most belonged seven predominant types, other 45 represented by few isolates. suggests that some these may have been introduced inward migration others result genetic changes one prevalent types. The divided into groups on basis sett at they captured, RFLP typing two or more 85 groups. Multiple identified among 50 groups, suggesting probably moved frequently between group territories.

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