摘要: The era of complete genome sequences has arrived and with it vast amounts data which must be annotated, cross referenced, placed within the regulatory networks define physiology an organism. One eucaryotic three procaryotic genomes have been completed made available another 50 are expected to by end decade. first steps in new post will decipher functions huge numbers open reading frames. Various approaches investigate what unknown genes do how interact together organism being undertaken, including (1) simultaneous measurement expression levels all a cell (2) mapping quantitation proteins expressed cell. idea systematically identifying total protein complement (the "proteome") arose over 20 years ago when separation from extracts two dimensional (2D) gel electrophoresis was developed. This review focus on use 2D as basis for constructing proteome maps rapid advances mass spectrometry allow large-scale, automated identification is necessary creation such databases.
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