Production of High-Titer RCAS Retrovirus

摘要: RCAS (B/P) is a replication-competent avian retrovirus engineered by Hughes et al. (J Virol 61:3004-3012, 1987) and referred to in this chapter as for simplicity. The has been widely used vehicle stable transduction of gene into cells both the developing chick embryo tissue/cell culture. It can be gain- loss-function experiments. ability virus spread among proliferating makes it possible achieve widespread retina. efficiency highly depending on titer viral stock, particularly experiments involving solid tissues such Here, we describe procedure that have 15 years generate stocks with 1-5 × 10(8) pfu/ml.