作者: A. J. Canty , L. M. Teles-Grilo Ruivo , C. Nesarajah , S. Song , J. S. Jackson
DOI: 10.1523/JNEUROSCI.0254-13.2013
关键词:
摘要: The axonal and synaptic mechanisms underlying dysfunction repair of the injured CNS are poorly understood. Unresolved issues include to what degree, when, how surviving neurons degenerate extent remodeling both along severed axon in nearby area. One main reasons is lack tools study complex asynchronous dynamic features individual lesioned responses intact brain. To address these issues, we combined two-photon microscopy laser microsurgery image real-time reorganization cortical circuitry at resolution for periods up 1 year brain living mice. Injured axons were eliminated proximally through a two-phase retraction process, which continued least 3 months postlesion was independent presence scar tissue. Remarkably, later attempt regenerate mature juvenile retracted less, raising possibility that targeting may improve chances regrowth after axotomy. Comparing prelesion dynamics on same over several days weeks revealed that, although synapse formation rates unaffected, boutons either rapidly persistently lost, or extremely resistant, depending cell-type their structural dynamics. Our data suggest lasting deficiency output surprising difference vulnerability axotomy, depend recent history.