Synaptic Elimination and Protection after Minimal Injury Depend on Cell Type and Their Prelesion Structural Dynamics in the Adult Cerebral Cortex

作者: A. J. Canty , L. M. Teles-Grilo Ruivo , C. Nesarajah , S. Song , J. S. Jackson

DOI: 10.1523/JNEUROSCI.0254-13.2013

关键词:

摘要: The axonal and synaptic mechanisms underlying dysfunction repair of the injured CNS are poorly understood. Unresolved issues include to what degree, when, how surviving neurons degenerate extent remodeling both along severed axon in nearby area. One main reasons is lack tools study complex asynchronous dynamic features individual lesioned responses intact brain. To address these issues, we combined two-photon microscopy laser microsurgery image real-time reorganization cortical circuitry at resolution for periods up 1 year brain living mice. Injured axons were eliminated proximally through a two-phase retraction process, which continued least 3 months postlesion was independent presence scar tissue. Remarkably, later attempt regenerate mature juvenile retracted less, raising possibility that targeting may improve chances regrowth after axotomy. Comparing prelesion dynamics on same over several days weeks revealed that, although synapse formation rates unaffected, boutons either rapidly persistently lost, or extremely resistant, depending cell-type their structural dynamics. Our data suggest lasting deficiency output surprising difference vulnerability axotomy, depend recent history.

参考文章(25)
BA McGuire, JP Hornung, CD Gilbert, TN Wiesel, Patterns of synaptic input to layer 4 of cat striate cortex The Journal of Neuroscience. ,vol. 4, pp. 3021- 3033 ,(1984) , 10.1523/JNEUROSCI.04-12-03021.1984
John C. Anderson, Kevan A. C. Martin, Does bouton morphology optimize axon length Nature Neuroscience. ,vol. 4, pp. 1166- 1167 ,(2001) , 10.1038/NN772
A. J. Canty, L. Huang, J. S. Jackson, G. E. Little, G. Knott, B. Maco, V. De Paola, In-vivo single neuron axotomy triggers axon regeneration to restore synaptic density in specific cortical circuits Nature Communications. ,vol. 4, pp. 2038- ,(2013) , 10.1038/NCOMMS3038
Anthony Holtmaat, Tobias Bonhoeffer, David K Chow, Jyoti Chuckowree, Vincenzo De Paola, Sonja B Hofer, Mark Hübener, Tara Keck, Graham Knott, Wei-Chung A Lee, Ricardo Mostany, Tom D Mrsic-Flogel, Elly Nedivi, Carlos Portera-Cailliau, Karel Svoboda, Joshua T Trachtenberg, Linda Wilbrecht, Long-term, high-resolution imaging in the mouse neocortex through a chronic cranial window Nature Protocols. ,vol. 4, pp. 1128- 1144 ,(2009) , 10.1038/NPROT.2009.89
Anna Letizia Allegra Mascaro, Leonardo Sacconi, Francesco S Pavone, Multi-Photon Nanosurgery in Live Brain Frontiers in Neuroenergetics. ,vol. 2, pp. 0- 0 ,(2010) , 10.3389/FNENE.2010.00021
Johanna Knöferle, Jan C Koch, Thomas Ostendorf, Uwe Michel, Véronique Planchamp, Polya Vutova, Lars Tönges, Christine Stadelmann, Wolfgang Brück, Mathias Bähr, Paul Lingor, Mechanisms of acute axonal degeneration in the optic nerve in vivo Proceedings of the National Academy of Sciences of the United States of America. ,vol. 107, pp. 6064- 6069 ,(2010) , 10.1073/PNAS.0909794107
Martin C Raff, Alan V Whitmore, John T Finn, Axonal self-destruction and neurodegeneration. Science. ,vol. 296, pp. 868- 871 ,(2002) , 10.1126/SCIENCE.1068613
Matthew J Farrar, Ida M Bernstein, Donald H Schlafer, Thomas A Cleland, Joseph R Fetcho, Chris B Schaffer, Chronic in vivo imaging in the mouse spinal cord using an implanted chamber Nature Methods. ,vol. 9, pp. 297- 302 ,(2012) , 10.1038/NMETH.1856
Thomas H Gillingwater, Cali A Ingham, Katherine E Parry, Ann K Wright, Jane E Haley, Thomas M Wishart, Gordon W Arbuthnott, Richard R Ribchester, Delayed synaptic degeneration in the CNS of Wlds mice after cortical lesion Brain. ,vol. 129, pp. 1546- 1556 ,(2006) , 10.1093/BRAIN/AWL101