Androgen Biosynthesis in Developing Ovarian Follicles Evidence that Luteinizing Hormone Regulates Thecal 17α-Hydroxylase and C17–2o-Lyase Activities*

摘要: In the pregnant rat, small antral follicles require increased LH activity to promote estradiol synthesis and preovulatory follicular development. To determine which steps in biosynthesis of activity, we compared abilities (from rats on day 16 pregnancy) 23 1) produce progesterone, androgen, (measured by RIA), 2) metabolize [3H]progesterone or [3H]17α-hydroxyprogesterone successive steroids Δ4- pathway. Although 6 ng progesterone during a 4-h incubation with hCG, 8-bromo-cAMP, human FSH, less than 50 pg testosterone were detected RIA same incubates. Small incubated presence [3H]progestins produced little authentic tritiated 5α-pregnan-3,20-dione, 3β-hydroxy-5α-pregnane-20-one, pregnan-20-one, 17α-hydroxyprogesterone, androstenedione, testosterone, estradiol. cont...