Curcumin reverses chemoresistance of human gastric cancer cells by downregulating the NF-κB transcription factor.

摘要: Gastric cancer remains one of the major health problems worldwide. Chemotherapy is an important therapeutic modality for gastric cancer, but success rate this treatment limited because chemoresistance. The ubiquitously expressed transcription factor NF-κB has been suggested to be associated with chemoresistance cancer. Agents that can either enhance effects chemotherapeutics or overcome are needed Curcumin, a component turmeric, such agent shown suppress and increase efficacy chemotherapy. In study, we investigated whether curcumin reverse by downregulating in human cells. SGC-7901 cells was treated (etoposide doxorubicin) combined application chemotherapeutics. viability measured MTT assay. Apoptosis detected using TUNEL Annexin V/PI methods. protein levels were analyzed immunocytochemical staining. EMSA used confirm increased nuclear translocation RelA. p-IκBα, Bcl-2 Bcl-xL Western blotting. suppressed growth cells, time-dose-dependent manner. Use addition these agents cell further (inhibitory rate: doxorubicin vs. + curcumin, 33% 45%, p<0.05; etoposide 35% 48%, p<0.05). Furthermore, induced apoptosis activated NF-κB. combination further, attenuated activation NF-κB, reduced expression NF-κB-regulated anti-apoptotic gene products Bcl-xL. Curcumin potentiates antitumor suppressing genes.