Prevalence and genetic diversity of norovirus genogroup II in children less than 5 years of age with acute gastroenteritis in Tehran, Iran.

摘要: Viral gastroenteritis is a major public health problem worldwide. In Iran, very limited studies have been performed with regard to the epidemiology of noroviruses. This study aimed evaluate prevalence and molecular GII noroviruses in hospitalized children less than 5 years age acute (AGE). A total 210 stool specimens were collected from Ali Asghar Children’s Hospital Bahrami Tehran, June 2015 2016. The samples screened by real-time RT-PCR for genogroup II (GII). Positive genotyped semi-nested PCR followed Sanger sequencing phylogenetic analysis. Norovirus was identified 36 (17.1%) specimens. Based on genetic analysis RdRp capsid sequences, strains clustered into eight RdRp–capsid genotypes: GII.P4–GII.4 Sydney_2012 (41.7%), GII.Pe–GII.4 (30.6%), GII.P21–GII.3 (13.9%), GII.P16–GII.4 (2.8%), GII.P16–GII.12 GII.P2–GII.4 GII.P7–GII.7 (2.8%) GII.P2–GII.2 (2.8%). We determined several different co-circulating norovirus genotypes children < 5 years AGE our hospital Iran. Continued surveillance noroviruses, including typing both genes, important monitoring emerging continued efforts reduce overall burden disease.