In vitro bone formation on a bone-like apatite layer prepared by a biomimetic process on a bioactive glass-ceramic.

摘要: In this study we have investigated the behavior of fetal rat osteoblasts, cultured up to 23 days, on a bioactive apatite-wollastonite (AW) glass-ceramic and same material which carbonated apatite layer had been formed by biomimetic process (AWa). At last day culture, specific activity alkaline phosphatase activity, as determined biochemically, was about 30% greater AWa compared with AW disks. After cell layers scraped off, scanning electron microscopic (SEM) observations materials' surfaces revealed that mineralized bone nodules remained attached both but in larger amounts AWa. X-ray microanalysis indicated presence calcium (Ca) phosphorus (P) tissue throughout surface Ca, P, silicon (Si) surface. The AW/ AWa/bone interfaces also were analyzed after fracturing interfacial analysis showed firm bonding surfaces, confirmed microanalytic mappings. These results indicate importance composition supporting differentiation osteogenic cells subsequent apposition matrix, allows strong bond materials bone. Furthermore, prefabrication biologic method mimics biomineralization could find application bone-repairing materials.