Plasticity of developing cerebellar cells in vitro studied with antibodies against the NG2 antigen.

摘要: The NG2 antigen, a chondroitin-sulfate proteoglycan, is cell surface marker for class of smooth protoplasmic astrocytes found throughout the brain and at high frequency in cerebellar molecular layer (Levine Card, 1987). To study development level single cell, we have analyzed distribution antigen by indirect immunofluorescence dissociated cultures prepared from postnatal cerebella compared that to several other intracellular antigens identify specific types nervous tissue. When cells 5 d rat pups were grown medium containing 10% fetal calf serum, NG2-labeled cells, which constituted 0.1-1.0% total glial present, contained fibrillary acidic protein (GFAP)-immunoreactive filaments bound monoclonal antibody A2B5, neurons some astrocytes. Approximately 30% also labeled with tetanus toxin, an additional immature Less than 2% antibodies against galactocerebroside or O1, both are markers oligodendrocytes. About half exhibited high-affinity uptake 3H-GABA, this was partially inhibited beta-alanine DABA. Thus, subpopulation type II fibrous present cultures. chemically defined, serum-free medium, had stellate morphology between 50-60% toxin. Although almost all less 5% expressed either oligodendrocyte GFAP immunoreactivity. As case serum-containing 60% 3H-GABA. However, DABA but not beta-alanine. This phenotype may be vitro analog NG2-labeled, filament-lacking, identified intact adult cerebellum. expression these 2 phenotypes could reversed switching tissue culture within plating cells. These results demonstrate environment can influence phenotypic properties developing suggest developmentally related O-2A lineage.