Cryopreservation and cell culture medium comprising less than 50 mM sodium ions and greater than 100 mM choline salt

摘要: A cell culture medium and cryopreservation in which sodium chloride is replaced with an organic cation, preferably choline a concentration of at least 100 mM, resulting residual ion less than about 50 mM. The solution suitable for unfertilized oocytes, thawed oocytes demonstrating the ability to survive, fertilize, embryos proceed full term development.