Electrochemical Immunosensors for the Rapid Screening of Cystic Fibrosis and Duchenne Muscular Dystrophy

摘要: Cystic Fibrosis (CF) and Duchenne Muscular Dystrophy (DMD) are well characterized progressive inherited diseases associated with significant morbidity mortality. Therefore, the early, rapid affordable diagnosis of these disorders through newborn screening is highly important for appropriate management. Here, we report label-free impedance immunosensors simple CF DMD detection cystic fibrosis transmembrane conductance regulator (CFTR) protein fragment a peptide sequence for dystrophin (DMD). The biosensors were constructed by covalent immobilization specific antibodies CFTR on standard gold (Au) electrodes. response was measured based change in electrochemical spectroscopy (EIS) signals after binding peptides. recognition immunosensor surfaces to target antigens leads retardation access ferri-ferrocyanide redox molecules surface thus, enhances charge transfer resistance (Rct). These impedimetric enabled sensitive, fast, selective accurate estimation levels within linear range from 1.0 pg/mL 1 μg/mL 10 ng/mL lower limits 0.8 0.7 pg/mL DMD, respectively. Moreover, tested human serum showing very good agreement enzyme-linked immunosorbent assays (ELISA). This work represents novel low cost analytical method that aims satisfy unmet public health need early can be extended detect other hereditary disorders.