Analysis of the upstream elements of the xenobiotic compound-inducible and positionally regulated glutathione S-transferase Ya gene.

摘要: In situ hybridization and other data showed that all hepatocytes express glutathione-S-transferase (GST) Ya mRNA but specifically pericentral cells can be induced 15- to 20-fold with 3-methylcholanthrene (3-MC). order identify DNA sequences involved in inducible expression (pericentral hepatocytes) constitutive (all hepatocytes), the upstream regions of GST gene were further analyzed by transient transfection DNA-binding studies nature proteins regulating this gene. The from -980 -650 necessary sufficient for cell-specific expression. Within enhancer region, four nuclear protein-binding sites identified. One site required was bound a protein(s) 3-MC. Two similar or identical hepatocyte factors HNF1 HNF4. fourth shown non-liver-specific protein is also important function albumin enhancer.