Simultaneous production of tumor necrosis factor-alpha and lymphotoxin by normal T cells after induction with IL-2 and anti-T3.

摘要: Normal human T cells purified from the peripheral blood and cultured for 6 days in presence of OKT3 IL-2 were shown to produce simultaneously TNF lymphotoxin (LT) mRNA. Both biologically active LT detected supernatants using WEHI 164 as target cells. The cytotoxic activities both cytokines could be inhibited with specific antibodies against or LT. cell cultures free macrophages, NK cells, B lymphocytes assessed by flow cytometric analysis.