EBP50, a β-catenin-associating protein, enhances Wnt signaling and is over-expressed in hepatocellular carcinoma
作者： Tatsuhiro Shibata , Makoto Chuma , Akiko Kokubu , Michiie Sakamoto , Setsuo Hirohashi
关键词:
摘要: Wnt signaling mediated by β-catenin plays crucial roles in the development of hepatocellular carcinoma and other cancers such as colorectal cancer. β-Catenin associates with T-cell factor (TCF) transcription factors functions a transcriptional activator nucleus. By protein interaction screening, we identified EBP50, cytoplasmic 2 PDZ domains, β-catenin-associating molecule. EBP50 interacted through its carboxyl-PDZ domain vitro vivo. Northern blot RT-PCR analysis revealed an increase messenger RNA (mRNA) (HCC) cell lines surgical specimens human HCC. Over-expression focal nuclear localization was detected In HCC cancer lines, enhanced β-catenin/TCF-dependent dose-dependent manner. line, over-expression carboxyl resulted decrease endogenous β-catenin/TCF transactivation. promoted β-catenin-mediated transactivation only cells which already stabilized, suggesting that may work stabilized for regulation. conclusion, EBP50/β-catenin complex promotes signaling, cooperatively liver
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