A method for the quantitation of hypericin, an antiviral agent, in biological fluids by high-performance liquid chromatography

摘要: Hypericin, a polycyclic aromatic dianthroquinone, is natural plant product with antiviral properties. We report here the development of methodology for extraction and quantitation hypericin from plasma biological fluids adaptation sensitive selective method detection compound by high-performance liquid chromatography. The offers rapid specific means monitoring drug blood levels in clinical pharmacokinetic studies. chromatographic procedure utilizes substantial retentive properties on reverse-phase media strong visible absorbance maximum at 590 nm. Verification fluorescence spectral organic can also be utilized. assay linear over 3 log concentration range consistently recovered murine, simian, human plasma. was applied to assess mice receiving single bolus injection 350 micrograms. A distribution half-life 2.0 h an elimination 38.5 were calculated. discuss limitations direct analysis or measurements.