Strategies for undertaking expressed sequence tag (EST) projects.

摘要: Complementary DNA (cDNA) sequencing can be used to sample an organism's transcriptome, and the generated EST sequences for a variety of purposes. They are especially important enhancing utility genome sequence or providing gene catalog that has not will sequenced. In planning executing cDNA project, several criteria must considered. One should clearly define project purpose, including organism tissue(s) choice, whether those tissues pooled, ability acquire adequate amounts clean well-preserved tissue, choice type(s) library, construction library (or libraries) is compatible with goals. addition, one possess skills construct libraries), keeping in mind number clones necessary meet requirements. If inexperienced construction, it might wise outsource production and/or analysis center company specializes activities. also aware new platforms being marketed may offer simpler protocols produce data more rapid economical manner. Of course, bioinformatics tools have place de-convolute aid these newer technologies. Possible funding sources projects include well-justified grant proposals, private funding, collaborators available funds.