Prior chemotherapy does not prevent effective mobilisation by G-CSF of peripheral blood progenitor cells.

摘要: In this study we demonstrate that the hemopoietic growth factor, G-CSF successfully mobilised progenitor cell populations into peripheral blood in a population of patients despite intensive pretreatment with chemotherapy. Administration increased numbers cells (PBPC) by median 76-fold above basal levels. Maximal levels PBPC were observed on days 5 and 6 after treatment. two second cycle to comparable those seen first An earlier (pre-CFC's) was also up 50-fold Using standard mononuclear leukapheresis technique collected extremely efficiently (essentially 100%) could be further enriched separation using Ficoll gradient. For who underwent optimal collection protocol (i.e. 5, 7) total 32 +/- x 10(4) GM-CFC kg-1 collected. The ability mobilise alone harvest these has important implications for future transplantation high dose chemotherapy procedures.