Internalization of C60 fullerenes into cancer cells with accumulation in the nucleus via the nuclear pore complex.
作者： Mustafa Raoof , Yuri Mackeyev , Matthew A. Cheney , Lon J. Wilson , Steven A. Curley
DOI: 10.1016/J.BIOMATERIALS.2011.12.043
关键词:
摘要: A highly water-soluble, non-ionic, and non-cytotoxic fullerene malonodiserinolamide-derivatized C60 (C60-ser) is under investigation as a potential nanovector to deliver biologic cancer drugs across biological barriers. Using laser-scanning confocal microscopy flow cytometry, we find that PF-633 fluorophore conjugated C60-ser nanoparticles (C60-serPF) are internalized within living cells in association with serum proteins through multiple energy-dependent pathways, escape endocytotic vesicles eventually localize accumulate the nucleus of nuclear pore complex. Furthermore, mouse model liver cancer, C60-serPF conjugate detected most tissues, permeating altered vasculature tumor tightly-regulated blood brain barrier while evading reticulo-endothelial system.
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