NOVEL SPOXAZOMICINS DERIVED FROM STREPTOMYCES SP. RM-14−6 ATTENUATE ETHANOL INDUCED CYTOTOXICITY IN VITRO

摘要: OF THESIS NOVEL SPOXAZOMICINS DERIVED FROM STREPTOMYCES SP. RM-14−6 ATTENUATE ETHANOL INDUCED CYTOTOXITY IN VITRO An estimated 13.9% of Americans currently meet criteria for an alcohol use disorder. Ultimately, chronic may result in neurological deficits, with up to 85% alcoholics exhibiting signs cognitive decline. However, biochemical and behavioral factors contributing this decline have remained elusive. Our ongoing research program encompasses a multi-tiered screening natural product library validation process provide novel information about mechanisms underlying these deficits identify chemical scaffolds be exploited the development pharmacological treatments disorders rodent organotypic hippocampal slice culture mode. Experiment 1 sought establish 48 h high throughput model testing against ethanol (EtOH) toxicity. 2 tested multiple compounds their ability attenuate ethanol-induced cytotoxicity. Results from revealed EtOH (100 mM) induced significant cytotoxicity at h. Trolox μM), potent antioxidant, was found reduce ethanolinduced assay. two spoxazomicins (1, 1-1) demonstrated cytoprotective effects These findings highlight potential applications treatment