Immunohistochemical staining of human teeth, and production of monoclonal antibodies against cementum
作者： Yasuyo Fukase
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摘要: Influence of various forms fixation and decalcification on immunohistochemical staining paraffin-embedded human teeth surrounding tissues was initially examined using commercially available antibodies against vimentin (V), type I collagen (C) cytokeratin (K). Secondly, monoclonal antibody (MoAb) produced both bovine cementum screening subsequently undertaken to test reactivity with different scheme decalcification. The combination neutral buffered paraformaldehyde fixation, Morse solution unmasking procedure yielded optimal morphology immunoreactivity. application this method into production MoAb or generated a variety MoAbs reactive tissues. percentage hybridoma supernatant sections 14.0-22.4% higher much improved compared results previous soft tissue studies. Finally, the MoAbs, BC 1 2 (isolated following use as immunogen) HC (human recognized specific bands molecular weight. three showed strong resistance periodate oxidation borohydrate reduction were stable treatment proteolytic enzymes. All immunoreactive components cementum.
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