Decoding the role of hypothetical protein All3255 of Anabaena PCC7120 in heavy metal stress management in Escherichia coli
作者： Prashant Kumar Singh , Mengmeng Tang , Sudhir Kumar , Alok Kumar Shrivastava
DOI: 10.1007/S00203-017-1462-2
关键词:
摘要: Cadmium is a non-essential toxic heavy metal for organisms, including plants and cyanobacteria. resistance transporters involved in of cells against various toxicants such as drugs effluxes cytotoxic compounds from cells. However, cadmium resistance-associated protein (CadD) has never been reported diazotrophic cyanobacterium Anabaena sp. To test whether the hypothetical All3255 PCC7120 homolog or not, cloning heterologous expression analysis all3255 performed Escherichia coli BL21 (DE3). Our results revealed that strain transformed with pGEX-5X-2 + all3255 showed resistant towards not only to but also other metals nickel, copper, zinc, lead cobalt addition arsenic than those empty vector (pGEX-5X-2). Furthermore, accumulation these unveil lower tested all3255-overexpressing E. vector. This study strongly supports role CadD efflux pump E.coli.
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