Adaptation and evaluation of three different in vitro tests for the detection of resistance to anthelmintics in gastro intestinal nematodes of cattle
作者： J. Demeler , U. Küttler , G. von Samson-Himmelstjerna
DOI: 10.1016/J.VETPAR.2010.01.032
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摘要: Abstract Three different in vitro methods, the Larval Development Test (LDT), Migration Inhibition (LMIT) and Micromotility Meter (MMT) have been adapted to detect anthelmintic resistance cattle nematodes. Nematode eggs third stage larvae of Ostertagia ostertagi Cooperia oncophora isolates were obtained from faecal cultures experimentally infected calves. Additionally, adult C. evaluated MMT for detection ivermectin (IVM). For all three tests standard operating procedures (SOPs) established successfully used responses non-parasitic parasitic stages substances description dose–response curves. In LDT (IVM) thiabendazole (TBZ) tested, LMIT IVM levamisole (LEV) only was evaluated. Susceptible O. ostertagi, an IVM-resistant isolate a TBZ-selected (C. oncophora) or some these (O. ostertagi). sigmoidal curves EC50 values tested using four-parameter logistic model. LDT, previously reported problem development overcome with mean rates between 80% 87% negative controls. Following optimization incubation times, temperatures, mesh sizes (LMIT only), nutritive medium (LDT only) group size (MMT test systems reliably detected significant differences response susceptible (p
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sci-hub.se 参考文章(44)
R. S. Rew, J. Vercruysse, Macrocyclic Lactones in Antiparasitic Therapy ,(2003)
M.A. TAYLOR, A larval development test for the detection of anthelmintic resistance in nematodes of sheep. Research in Veterinary Science. ,vol. 49, pp. 198- 202 ,(1990) , 10.1016/S0034-5288(18)31077-4
J. F. Michel, The regulation of egg output by Ostertagia ostertagi in calves infected once only. Parasitology. ,vol. 59, pp. 767- 774 ,(1969) , 10.1017/S003118200007030X
V.H. Suarez, S.L. Cristel, Anthelmintic resistance in cattle nematode in the western Pampeana Region of Argentina Veterinary Parasitology. ,vol. 144, pp. 111- 117 ,(2007) , 10.1016/J.VETPAR.2006.09.016
TS Waghorn, DM Leathwick, AP Rhodes, R Jackson, WE Pomroy, DM West, JR Moffat, Prevalence of anthelmintic resistance on 62 beef cattle farms in the North Island of New Zealand. New Zealand Veterinary Journal. ,vol. 54, pp. 278- 282 ,(2006) , 10.1080/00480169.2006.36711
B. Rabel, R. Mcgregor, P.G.C. Douch, Improved bioassay for estimation of inhibitory effects of ovine gastrointestinal mucus and anthelmintics on nematode larval migration International Journal for Parasitology. ,vol. 24, pp. 671- 676 ,(1994) , 10.1016/0020-7519(94)90119-8
D.J. Giordano, J.P. Tritschler, G.C. Coles, Selection of ivermectin-resistant Trichostrongylus colubriformis in lambs Veterinary Parasitology. ,vol. 30, pp. 139- 148 ,(1988) , 10.1016/0304-4017(88)90161-6
Jennifer H. Gill, Judith M. Redwin, Jan A. Van Wyk, Ernest Lacey, Avermectin inhibition of larval development in Haemonchus contortus — Effects of ivermectin resistance International Journal for Parasitology. ,vol. 25, pp. 463- 470 ,(1995) , 10.1016/0020-7519(94)00087-5
M.A. Taylor, K.R. Hunt, K.L. Goodyear, Anthelmintic resistance detection methods. Veterinary Parasitology. ,vol. 103, pp. 183- 194 ,(2002) , 10.1016/S0304-4017(01)00604-5
N.C. Sangster, F.L. Riley, G.H. Collins, Investigation of the mechanism of levamisole resistance in trichostrongylid nematodes of sheep International Journal for Parasitology. ,vol. 18, pp. 813- 818 ,(1988) , 10.1016/0020-7519(88)90123-3