Pharmacokinetics of Schizandrin and Its Pharmaceutical Products Assessed Using a Validated LC–MS/MS Method

摘要: Schisandra chinensis has been used as an important component in various prescriptions traditional Chinese medicine and, more recently, Western-based for its anti-hepatotoxic effect. The aim of this study was to develop a selective, rapid, and sensitive ultra-performance liquid chromatography-tandem mass spectrometry method pharmacokinetic studies schizandrin rats. Liquid-liquid extraction plasma sample preparation. A UHPLC reverse-phase C18e column (100 mm × 2.1 mm, 2 μm) coupled with mobile phase methanol-0.1% formic acid (85:15, v/v) separation. triple quadrupole tandem spectrometer detect the analytes selected reaction monitoring mode. linear range rat 5.0–1000 ng/mL (r2 > 0.999), lower limit quantification 5 ng/mL. validated regard accuracy, intra-day inter-day precision, linearity, stability, recovery, matrix effects plasma, which were acceptable according biological validation guidelines developed by FDA. This successfully applied after oral administration 3 g/kg 10 products, yielded maximum concentration 0.08 ± 0.07 0.15 0.09 μg/mL, respectively. parallel design investigate bioavailability single compound herbal extract, pure (10 mg/kg, i.v.), p.o.), extract (3 g/kg, p.o.) given individually. dose g/kg) equivalent (5.2 mg/kg); (17.3 mg/kg). result demonstrated that approximately 15.56 10.47% rats, however higher than compound. pharmaceutical industry products