Uptake of phenol by Trichosporon cutaneum.

摘要: The soil yeast Trichosporon cutaneum, which is distinguished by having a strictly oxidative metabolism, can be induced to utilize phenol as sole carbon source. present paper shows that such phenol-induced cells contain specific, energy-dependent uptake system for phenol. Phenol not directly linked its o-hydroxylation inside the cell, first step of metabolism. Km 235 +/- 30 microM, hydroxylation only 4.5 0.5 microM. Further, analog 2,6-dimethylphenol, hydroxylated, competes with system. pH dependence indicates phenolate an essential form during process. energy requirement indicated effects various inhibitors generation, including proton-conducting uncouplers. Direct monitoring proton movements in pH-stat phenol-proton symport. One cotransported every molecule. sucrose and glycerol grown on these substrates. Under conditions seems proceed through different system, lower affinity than phenol-grown cells.