Efficacy of the Antiadhesin Octyl O-(2-Acetamido-2-Deoxy-β-d- Galactopyranosyl)-(1-4)-2-O-Propyl-β-d-Galactopyranoside (Fimbrigal-P) in a Rat Oral Candidiasis Model

摘要: Adherence of Candida albicans to buccal epithelial cells via its fimbrial subunit requires the minimal disaccharide sequence beta-GalNAc(1-4)-beta-galactosidase in host cell receptors asialo-GM1 or asialo-GM2. This and other disaccharides some synthetic derivatives have been shown inhibit purified pathogen binding vitro. study evaluates vivo efficacy propyl derivative this disaccharide, octyl O-(2-acetamido-2-deoxy-beta-D-galactopyranosyl)-(1-4)-2-O-propyl-beta-D-galactopyranoside, Fimbrigal-P, incorporated into a mucoadhesive polymer formulation rat oral candidiasis model. Colony counts microcurette samples from cavity tongue homogenates were used estimate effectiveness four treatment modalities reduce fungal burden. All (preventative, premixing with inoculant, drinking water, treatment) significantly reduced burden compared untreated control animals by day 9; however, preventative pre-mixing approaches provided faster rate clearance. The low toxicity immunogenicity carbohydrate stability saliva, as demonstrated high-performance liquid chromatography, make it promising candidate for prevention microbial infections which relies on establish adherence.