Systemic injection of neural stem/progenitor cells in mice with chronic EAE.

摘要: Neural stem/precursor cells (NPCs) are a promising stem cell source for transplantation approaches aiming at brain repair or restoration in regenerative neurology. This directive has arisen from the extensive evidence that is achieved after focal systemic NPC several pre clinical models of neurological diseases. These experimental data have identified delivery route as one main hurdles restorative therapies diseases requires urgent assessment. Intraparenchymal grafting represents logical approach to those pathologies characterized by isolated and accessible lesions such spinal cord injuries Parkinson’s disease. Unfortunately, this principle poorly applicable conditions multifocal, inflammatory disseminated (both time space) nature, including multiple sclerosis (MS). As such, targeting become low invasive, therapeutically efficacious protocol deliver rodents non human primates affected chronic damage central nervous system (CNS). alternative method relies on pathotropism, specifically their innate capacity (i) sense environment via functional adhesion molecules cytokine chemokine receptors; (ii) cross leaking anatomical barriers intravenous (i.v.) intracerebroventricular (i.c.v.) injection; (iii) accumulate level perivascular site(s) damage; exert remarkable tissue trophic immune regulatory effects onto different host target vivo. Here we describe methods developed i.v. i.c.v. syngeneic NPCs mice with autoimmune encephalomyelitis (EAE), model CNS demyelination, envisage valuable technique selective inflamed