Effect of Anisakis simplex (sl) larvae on the spoilage rate and shelf-life of fish mince products under laboratory conditions

摘要: Wild caught marine fish are commonly infected with anisakid nematodes lodging in the intestinal linings or muscle. One of most found nematode parasites is Anisakis simplex. During production mince from muscle wild Anisakis-infected fish, larvae would be disrupted during production. Any bacteria within on surface such mincing process evenly distributed throughout mince, and could thus possibly affect spoilage rate final products. To explore if how any associated muscle-invading may a controlled storage trial was conducted. Fillets farmed Atlantic cod (Gadus morhua), exclusively fed dried heat-treated compound feed hence expectably free larvae, were aseptically collected homogenised. Fish aliquots added different volumes homogenate based which freshly sampled visceral cavity NE blue whiting (Micromesistius poutassou). The parasite (parasite(þ)-samples) reflected infection intensities 15 (low) to 50 (high) per 100 g fillet, representing an actual intensity range flesh whiting. samples kept at 4 � C for days subjected microbiological, sensory chemical evaluation 3 intervals. Upon visual examination plate count measurements (PC) Iron Agar Lyngby (IAL), without additives (no[parasite]) spoiled differently more rapidly than parasite(þ)-samples. However, H2S-producing only recorded latter samples, also ones that showed increased levels indicator substance trimethylamine (TMA). Moreover, parasite(þ)-samples changed their characteristics later stage compared no[parasite]-samples. Although some cultures H2Sproducing IAL, molecular identification by PCR-DGGE not conclusive. Psychrobacter sp. has no little activity, identified all until day 9, but probably outgrown stronger spoilers Pseudomonas fluorescence/fragi Photobacterium phosphoreum. Thus, somewhat unexpected, our findings indicate e under present conditions contaminated originate less parasite-related present. shelf-life apparently reduced presence transferred larvae.