Method for the production of vitamin a
作者： Johannes Lintig , Klaus Vogt
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摘要: The present invention provides means and methods of transforming bacteria, fungi including yeast, animal plant cells, seeds, tissues whole plants in order to yield transformants capable expressing a β-carotene (15, 15') dioxygenase accumulating vitamin A aldehyde. further biotechnically produce retinoids using tissues, organs or organisms which natively after transformation accumulate take up from the medium. also DNA molecules encoding dixoygenases derived different sources taxonomic groups living designed be suitable for carrying out method invention, plasmids vector systems comprising said molecules. Furthermore, transgenic that display an improved nutritional quality physiological condition contain such and/or have been generated by use invention.
lens.org 参考文章(5)
A. Wyss, G. Wirtz, W.-D. Woggon, R. Brugger, M. Wyss, A. Friedlein, H. Bachmann, W. Hunziker, Cloning and Expression of β,β-Carotene 15,15′-Dioxygenase Biochemical and Biophysical Research Communications. ,vol. 271, pp. 334- 336 ,(2000) , 10.1006/BBRC.2000.2619
Johannes von Lintig, Klaus Vogt, Filling the Gap in Vitamin A Research MOLECULAR IDENTIFICATION OF AN ENZYME CLEAVING β-CAROTENE TO RETINAL Journal of Biological Chemistry. ,vol. 275, pp. 11915- 11920 ,(2000) , 10.1074/JBC.275.16.11915
Johannes von Lintig, Armin Dreher, Cornelia Kiefer, Mathias F Wernet, Klaus Vogt, Analysis of the blind Drosophila mutant ninaB identifies the gene encoding the key enzyme for vitamin A formation in vivo Proceedings of the National Academy of Sciences of the United States of America. ,vol. 98, pp. 1130- 1135 ,(2001) , 10.1073/PNAS.031576398
Jannine Devery, B. V. Milborrow, β-Carotene-15,15′-dioxygenase (EC 1.13.11.21) isolation reaction mechanism and an improved assay procedure British Journal of Nutrition. ,vol. 72, pp. 397- 414 ,(1994) , 10.1079/BJN19940042
Arno Martin Friedlein, Adrian Wyss, Markus Wyss, Gabriele Margarethe Wirtz, Heinrich Bachmann, Wolf-Dietrich Woggon, Roland Brugger, Protein having enzymatic activity in the cleavage of beta-carotene ,(2000)