Using the power of genetic suppressors to probe the essential functions of RNase E
作者： Diarmaid Hughes
DOI: 10.1007/S00294-015-0510-1
关键词:
摘要: This review describes how, using the power of genetic suppressor analysis, mRNA turnover in bacteria was shown to be an essential function RNase E. E is multifunctional enzyme bacteria, involved processing stable RNAs their mature forms (rRNAs and tRNAs) most mRNAs. Genetic analysis successfully used address whether one functions Conditional lethal mutations rne were suppressible by three different classes extragenic suppressors, including a class that caused overexpression RelE. The only known RelE cleavage ribosomal A-site. Suppression conditional defect provides strong evidence Several hypotheses could explain why are discussed. Suppressor old-fashioned but very powerful approach can usefully applied wide variety important questions biology genetics. In this work has revealed E, conclusion raises host interesting for future research.
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