Protective effects of coffee‐derived compounds on lipopolysaccharide/d‐galactosamine induced acute liver injury in rats

摘要: Objectives The protective effects of coffee-derived compounds on lipopolysaccharide/d-galactosamine (LPS/d-GalN) induced acute liver injury in rats were investigated. Methods Wistar orally administered saline (control) or one the test (caffeine, chlorogenic acid, trigonelline, nicotinic acid eight ***pyrazinoic acids) at a dose 100 mg/kg, respectively. This was followed by intraperitoneal injection with LPS (100 μg/kg)/d-GalN (250 mg/kg) 1 h after administration compounds. Blood samples collected up to 12 LPS/d-GalN injection, determination plasma aspartate aminotransferase, alanine tumour necrosis factor α (TNF-α) and interleukin 10 (IL-10) levels. Key findings Plasma aminotransferase levels significantly increased LPS/d-GalN-treatment, but suppressed pretreatment caffeine (n = 5), non-substituted pyrazinoic 5-methylpyrazinoic 6, respectively) LPS/d-GalN-treatment (P < 0.01, respectively). Moreover, animals pretreated these showed higher survival rates (83–100%) compared control (23%). Only elevation TNF-α 2 Pretreatment caffeine, activated IL-10 h, although there no statistically significant differences between treated rats. Conclusions results suggest that can protect against injury, which may be mediated reduction production and/or increasing production.