Temperature dependence of growth and membrane-bound activities of Chloroflexus aurantiacus energy metabolism.

摘要: The temperature dependence of various activities related to the energy metabolism isolated membranes and whole cells thermophilic bacterium Chloroflexus aurantiacus was determined after phototrophic growth at either 40, 50, or 60 degrees C. data obtained were expressed by use Arrhenius plots. Maximum about 65 C for succinate 2,4-dichlorophenol-indophenol reductase as well NADH oxidase 70 Mg-ATPase light-induced proton extrusion cells. Activation energies 40 kJ mol-1 from 30 50 they increased significantly higher temperatures. Essentially same dependency detectable with oxidase, except an increase in activation below 41 All these responses independent temperature. Succinate-2,4-dichlorophenol-indophenol showed a change around only grown Differences different temperatures identified on basis changes sigmoidal hyperbolic kinetics light saturation extrusion. Moreover, thermostability maximal when assayed corresponding In any case, lowest 68 assay Differential scanning calorimetry revealed irreversible heat uptake 72 results are discussed specific rate, which is optimum