`Affinity proteomics to study endogenous protein complexes: Pointers, pitfalls, preferences and perspectives
作者： John LaCava , Kelly R. Molloy , Martin S. Taylor , Michal Domanski , Brian T. Chait
DOI: 10.2144/000114262
关键词:
摘要: Dissecting and studying cellular systems requires the ability to specifically isolate distinct proteins along with co-assembled constituents of their associated complexes. Affinity capture techniques leverage high affinity, specificity reagents target interest from cell extracts. coupled mass spectrometry (MS)-based proteomic analyses has enabled isolation characterization a wide range endogenous protein Here, we outline effective procedures for affinity complexes, highlighting best practices common pitfalls.
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