Hyaluronan Synthesis in Virus PBCV-1-Infected Chlorella-like Green Algae

摘要: We previously reported that the chlorella virus PBCV-1 genome encodes an authentic, membrane-associated glycosyltransferase, hyaluronan synthase (HAS). Hyaluronan, a linear polysaccharide chain composed of alternating beta1,4-glucuronic acid and beta1, 3-N-acetylglucosamine groups, is present in vertebrates as well few pathogenic bacteria. Studies infected cells show transcription has gene begins within 10 min infection ends at 60-90 postinfection. The to accumulate hyaluronan-lyase sensitive, hair-like fibers on outside cell wall by 15-30 postinfection; 240 postinfection, are coated with dense fibrous network. This slightly reduces attachment second algae. An analysis 41 additional viruses indicates many, but not all, produce during infection.