Use of a fluorescent probe to monitor the enhanced affinity of rh-BMP-2 to silicated-calcium phosphate synthetic bone graft substitutes under competitive conditions.

摘要: Abstract A comparative investigation was undertaken on 1–2 mm sized granules of two forms synthetic bone graft substitute (SBG) with identical pore structure but varied bulk chemistry, stoichiometric hydroxyapatite (HA) and silicate substituted (0.8 wt% Si) (SA), to assess the influence SBG chemistry relative affinity an osteogenic growth factor (GF), recombinant human morphogenetic protein-2 (rhBMP-2). previously described novel fluorescent probe, fluoresceinthioureidoaminocaproic acid (FTCA), covalently attached rhBMP-2 give FTCA-rhBMP-2 facilitate quantitative monitoring GF uptake release from chemistries SBG. The for HA SA assessed at a physiologically relevant concentration 300 ng mL − 1 three (increasingly complex) environments; phosphate buffered saline (PBS), minimum Eagles' medium (MEM) serum supplemented MEM (SCEM) in order closely mimic clinical repair procedures. results demonstrated that SBGs highly sensitive both composition local environment. Under most competitive conditions SCEM, showed greater ( P