Antibody-based tissue profiling as a tool for clinical proteomics
作者： Fredrik Ponten , Caroline Kampf , Ann-Catrin Andersson , Kenneth Wester , Erik Björling
DOI: 10.1385/CP:1:3-4:285
关键词:
摘要: Here, we show a strategy for high-throughput antibody-based tissue profiling with the aim to create an atlas of protein expression patterns in normal human tissues and cancer representing 20 most prevalent types. A set standardized microarrays (TMAs) was produced allow rapid screening multitude different cells using immunohistochemistry. Eight TMA blocks were containing 48 triplicate from 216 individually tumors duplicate. Sections these immunohistochemically stained five commercial in-house generated antibodies. Digital images annotation profiles semiautomated approach. Five hundred seventy-six data corresponding total 30 Gbytes collected each antibody. The presented here suggest that can be used as valuable tool clinical proteomics.
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Rimm Dl, Costa J, Camp Rl, Charette La, Reiss M, Olsen Da, Tissue microarray: a new technology for amplification of tissue resources. Cancer Journal. ,vol. 7, pp. 24- 31 ,(2001)
Åsa Sivertsson, Anna Asplund, Helena Bäckvall, Joakim Lundeberg, Fredrik Ponten, Sara Strömberg, Mutational Spectra of p53 Clones adjacent to Basal Cell Carcinoma and Squamous Cell Carcinoma Experimental Dermatology. pp. 1663- ,(2004)
John Quackenbush, Computational analysis of microarray data Nature Reviews Genetics. ,vol. 2, pp. 418- 427 ,(2001) , 10.1038/35076576
Till Braunschweig, Joon-Yong Chung, Stephen Hewitt, Perspectives in tissue microarrays. Combinatorial Chemistry & High Throughput Screening. ,vol. 7, pp. 575- 585 ,(2004) , 10.2174/1386207043328445
Michael J Becich, None, The Role of the Pathologist as Tissue Refiner and Data Miner: The Impact of Functional Genomics on the Modern Pathology Laboratory and the Critical Roles of Pathology Informatics and Bioinformatics Molecular Diagnosis. ,vol. 5, pp. 287- 299 ,(2000) , 10.1007/BF03262090
Susan L. Abbondanzo, Paraffin immunohistochemistry as an adjunct to hematopathology Annals of Diagnostic Pathology. ,vol. 3, pp. 318- 327 ,(1999) , 10.1016/S1092-9134(99)80030-6
Kenneth Wester, Ann-Catrin Andersson, Petter Ranefall, Ewert Bengtsson, Per-Uno Malmstr�m, Christer Busch, Cultured human fibroblasts in agarose gel as a multi-functional control for immunohistochemistry : Standardization Of Ki67 (MIB1) assessment in routinely processed urinary bladder carcinoma tissue The Journal of Pathology. ,vol. 190, pp. 503- 511 ,(2000) , 10.1002/(SICI)1096-9896(200003)190:4<503::AID-PATH537>3.0.CO;2-E
Robert L. Camp, Gina G. Chung, David L. Rimm, Automated subcellular localization and quantification of protein expression in tissue microarrays. Nature Medicine. ,vol. 8, pp. 1323- 1327 ,(2002) , 10.1038/NM791
Wen-Hui Wan, Michael B. Fortuna, Philip Furmanski, A rapid and efficient method for testing immunohistochemical reactivity of monoclonal antibodies against multiple tissue samples simultaneously Journal of Immunological Methods. ,vol. 103, pp. 121- 129 ,(1987) , 10.1016/0022-1759(87)90249-3
Charlotta Agaton, Ronny Falk, Ingmarie Höidén Guthenberg, Lovisa Göstring, Mathias Uhlén, Sophia Hober, Selective enrichment of monospecific polyclonal antibodies for antibody-based proteomics efforts. Journal of Chromatography A. ,vol. 1043, pp. 33- 40 ,(2004) , 10.1016/J.CHROMA.2004.06.008