CRISPR elements and their association with antimicrobial resistance and virulence genes among vancomycin-resistant and vancomycin-susceptible enterococci recovered from human and food sources
作者： Rachel Leite Ribeiro , Lúcia Martins Teixeira , Felipe Piedade Gonçalves Neves , Barbara Araújo dos Santos , Jessica da Silva de Oliveira
DOI: 10.1016/J.MEEGID.2020.104183
关键词:
摘要: Abstract We aimed to investigate the occurrence of CRISPR elements in genomes vancomycin-resistant (VRE) and vancomycin-susceptible (VSE) enterococci their association with presence antimicrobial resistance virulence genes. analyzed 180 isolates, including 91 VRE 89 VSE. Isolates were identified by PCR or MALDI-TOF. Antimicrobial susceptibility MICs for vancomycin determined disk-diffusion method E-test®, respectively. The genes, as well elements, was investigated PCR. 95 (53%) E. faecalis, 78 (43%) faecium, five (2.8%) gallinarum, one (0.6% each) casseliflavus durans. highest lowest non-susceptibility frequencies observed erythromycin (n = 152; 84.4%) fosfomycin (n = 5; 2.8%), Most erythromycin-resistant isolates had erm(B) gene (106/152; 69.7%). Of 118 (65.6%) high-level aminoglycoside, 69 (58.5%) at least aminoglycoside gene, mostly ant(6)-Ia aac(6′)-Ie + aph(2″)-Ia. found among 135 (75%) gelE (79/180; 43.9%). Ninety-two (51.1%) element, especially CRISPR3 (62/92; 67.4%). more common which we a relationship between absence vanA hyl esp Among VRE. gene. In conclusion, evident associations lack species, multidrug resistance, major resistance- virulence-associated
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