Pro-angiogenic character of endothelial cells and gingival fibroblasts cocultures in perfused degradable polyurethane scaffolds.

摘要: Gingival atrophy manifests as exposure of the tooth root surface because recession gingiva, a condition that affects >20% adults and leads to increased sensitivity ultimately, loss. Tissue engineering approaches employ novel synthetic polymeric scaffolds are being considered for rebuilding gingival lamina propria lost in atrophic process. Specifically, polyurethane hydrogels (degradable/polar/hydrophobic/ionic [D-PHI]) can enhance proliferation human fibroblasts (HGFs) collagen production perfusion system. However, few studies have assessed potential block copolyurethanes initiate blood vessel formation an vitro bioreactor As is highly vascular, coculture system umbilical vein endothelial cells (HUVECs) with HGFs was used perfused D-PHI determine feasibility initiating vascularization. Culture conditions were optimized driving cocultures toward desired tissue-engineered construct. HUVEC-HGF cell seeding density at least 80,000 cells/scaffold 50/50 mix HUVEC HGF media (by volume) exhibited enhanced growth vascular factor fibroblast (FGF)-2 production, well reduced myofibroblast differentiation. A greater proportion (seeding ratio 1:2) resulted cluster formations transforming factor-β1 FGF-2 production. The combined pro-angiogenic effects provided by these culture anticipated be important development vascularized construct regenerating possibly other soft tissues.