Strategies for facilitated protein recovery after recombinant production in Escherichia coli
作者： My Hedhammar
DOI:
关键词:
摘要: The successful genomic era has resulted in a great demand for efficient production and purification of proteins. main objective the work described this thesis was to develop methods fa ...
参考文章(336)
Chad A. Galloway, Mark P. Sowden, Harold C. Smith, Increasing the yield of soluble recombinant protein expressed in E. coli by induction during late log phase. BioTechniques. ,vol. 34, pp. 524- 530 ,(2003) , 10.2144/03343ST04
Karen M. Routzahn, David S. Waugh, Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins. Journal of Structural and Functional Genomics. ,vol. 2, pp. 83- 92 ,(2002) , 10.1023/A:1020424023207
Amulya K. Panda, Bioprocessing of therapeutic proteins from the inclusion bodies of Escherichia coli. Advances in Biochemical Engineering \/ Biotechnology. ,vol. 85, pp. 43- 93 ,(2003) , 10.1007/3-540-36466-8_3
Marcus Mayer, Johannes Buchner, Refolding of Inclusion Body Proteins Molecular Diagnosis of Infectious Diseases. ,vol. 94, pp. 239- 254 ,(2004) , 10.1385/1-59259-679-7:239
Urs von Stockar, Luuk van der Wielen, Sven-Olaf Enfors, Process integration challenges in biotechnology yesterday, today and tomorrow. Advances in Biochemical Engineering \/ Biotechnology. ,vol. 80, ,(2003)
W. Kauzmann, Some factors in the interpretation of protein denaturation. Advances in Protein Chemistry. ,vol. 14, pp. 1- 63 ,(1959) , 10.1016/S0065-3233(08)60608-7
Dolores J. Cahill, Eckhard Nordhoff, Protein arrays and their role in proteomics. Advances in Biochemical Engineering \/ Biotechnology. ,vol. 83, pp. 177- 187 ,(2003) , 10.1007/3-540-36459-5_7
A Plückthun, A Knappik, An improved affinity tag based on the FLAG peptide for the detection and purification of recombinant antibody fragments BioTechniques. ,vol. 17, pp. 754- 761 ,(1994)
O B Ptitsyn, [Stages in the mechanism of self-organization of protein molecules]. Proceedings of the USSR Academy of Sciences. ,vol. 210, pp. 1213- 1215 ,(1973)
Juan A. Asenjo, Separation Processes in Biotechnology ,(1990)