Production of Polyhydroxyalkanoates (PHAs) by Bacillus Strain Isolated from Waste Water and Its Biochemical Characterization

摘要: Municipal sewage triggers a stress prone environment to accumulate polyhydroxyalkanoates (PHAs) in the cytosol of bacteria. In view that, different Bacillus species were isolated from municipal and screened for evaluating their efficacy PHA production. Growth parameters such as temperature, pH, glucose concentration carbon nitrogen combinations optimized with respect higher biomass production it is analogous accumulation. Under conditions, produced 3.09 g/L PHAs which was estimated yield comparison other similar strains. Fourier transform infrared spectroscopic analysis extracted polyhydroxybutyrate confirmed distinct peak corresponding C=O group, whereas proton nuclear magnetic resonance (1H NMR) differential scanning colorimetric exhibited detailed insight its chemical structure properties by reflecting monomeric unit. The high yielding bacterial isolate identified 16S rDNA sequencing sequence subtilis an accession no. KP172548 after submission NCBI data base. potential bacterium may be further exploited cost effective mass scale biopolymer.