Methods of Permeabilization

摘要: Viable cells are capable of living, developing and reproducing themselves. Under experimental conditions the parameters viability diverse measured by different tests, redox potential, integrity cell membranes, cellular activities, enzymes. Each assay shows a angle may provide basis assays related to cytotoxicity. Non-invasive treatment maintain have received wide attention remained one major challenges facing reparative medicine. Biopreservation is most important processes in maintaining functionality held outside native environment for extended storage times. The importance preservation exemplified long-term red blood (RBCs), critical ensure safe supply transfusion medicine (Scott et al, Transfus Med Rev 19:127–142, 2005). This chapter describes manipulations that all aim make permeable while not only structural but also functional cells. Unfortunately there no unique, fast, reliable solution permeabilization could be general use. discussion methods will include advantages disadvantages these methods.