Phosphodiesterase (PDE5) inhibition assay for rapid detection of erectile dysfunction drugs and analogs in sexual enhancement products

摘要: Products marketed as dietary supplements for sexual enhancement are frequently adulterated with phosphodiesterase-5 (PDE5) inhibitors, which erectile dysfunction drugs or their analogs that can cause adverse health effects. Due to widespread adulteration, a rapid screening assay was developed detect PDE5 inhibitors in products. The employs fluorescence detection and is based on measuring inhibition of activity, the pharmacological mechanism shared among adulterants. Initially, reaction scheme established characterized, followed by analysis 9 representative (IC50 , 0.4-4.0 ng mL-1 ), demonstrating sensitive matrix-free solutions. Next, serving matrix blanks (n = 25) were analyzed determine interference establish threshold value; there no false positives. Finally, spiked individual along several mixtures. All adulterants successfully detected (≤ 5 % negative rate; n 20) at concentration 1.00 mg g-1 over times lower than concentrations commonly encountered A major distinction ability without prior knowledge chemical structures, broad-based capability address continuously evolving threat new analyze 40 samples simultaneously within 15 minutes involves single incubation step simple data analysis, all advantageous combating adulteration sex-enhancement