Cloning yeast telomeres on linear plasmid vectors

摘要: We have constructed a linear yeast plasmid by joining fragments from the termini of Tetrahymena ribosomal DNA to vector. Structural features terminus region rDNA maintained in include set specifically placed single-strand interruptions within cluster hexanucleotide (C4A2) repeat units. An artificially hairpin was unable stabilize yeast. The fact that can recognize and use ends distantly related organism suggests structural required for telomere replication resolution been highly conserved evolution. used as vector clone chromosomal telomeres One end removed restriction digestion, could function an on were selected. Restriction mapping hybridization analysis demonstrated these telomeres, suggested all chromosomes might common sequence. Yeast appear be similar structure Tetrahymena, which specific nicks or gaps are present simple repeated sequence near DNA.