Fusion expression of human beta-defensin-2 from multiple joined genes in Escherichia coli.

作者: Fang Wang , Xiangming Fang , Zhinan Xu , Li Peng , Peilin Cen

DOI: 10.1081/PB-200026797

关键词:

摘要: … coli BL21 (DE3) was used as a host strain for expression. … The expression vector, pET28a‐hBD2 was constructed through a … Following the same procedure, the expression vectors …

参考文章(11)
Tomas Ganz, Defensins and Host Defense Science. ,vol. 286, pp. 420- 421 ,(1999) , 10.1126/SCIENCE.286.5439.420
J. Harder, J. Bartels, E Christophers, J.-M. Schröder, A peptide antibiotic from human skin Nature. ,vol. 387, pp. 861- 861 ,(1997) , 10.1038/43088
Xu Zhinan, Liu Gang, Cen Peilin, W. K. R. Wong, Factors influencing excretive production of human epidermal growth factor (hEGF) with recombinant Escherichia coli K12 system Bioprocess Engineering. ,vol. 23, pp. 669- 674 ,(2000) , 10.1007/S004490000218
Kevin L. Piers, Melissa H. Brown, Robert E.W. Hancock, Recombinant DNA procedures for producing small antimicrobial cationic peptides in bacteria Gene. ,vol. 134, pp. 7- 13 ,(1993) , 10.1016/0378-1119(93)90168-3
J. Raingeaud, F. Lavergne, V. Lelievre, J.M. Muller, R. Julien, Y. Cenatiempo, Production, analysis and bioactivity of recombinant vasoactive intestinal peptide analogs. Biochimie. ,vol. 78, pp. 14- 25 ,(1996) , 10.1016/0300-9084(96)81324-1
Jens-M Schröder, Jürgen Harder, Human beta-defensin-2. The International Journal of Biochemistry & Cell Biology. ,vol. 31, pp. 645- 651 ,(1999) , 10.1016/S1357-2725(99)00013-8
Xiangming Fang, Li Peng, Zhinan Xu, Jinmin Wu, Peilin Cen, Cloning and Expression of Human Beta-defensin-2 Gene in Escherichia Coli Protein and Peptide Letters. ,vol. 9, pp. 31- 37 ,(2002) , 10.2174/0929866023409011
S C Makrides, Strategies for achieving high-level expression of genes in Escherichia coli. Microbiological Research. ,vol. 60, pp. 512- 538 ,(1996) , 10.1128/MR.60.3.512-538.1996
J Hackett, R.C Huang, E Lam, T.L Lam, K.L Siu, R.S.C Wong, W.K.R Wong, S Sivakesava, Z.N Xu, Y.H Chen, Production of excreted human epidermal growth factor (hEGF) by an efficient recombinant Escherichia coli system Process Biochemistry. ,vol. 34, pp. 893- 900 ,(1999) , 10.1016/S0032-9592(99)00013-8