Polygonatum cyrtonema lectin induces apoptosis and autophagy in human melanoma A375 cells through a mitochondria-mediated ROS–p38–p53 pathway
作者： Bo Liu , Yan Cheng , Bo Zhang , He-jiao Bian , Jin-ku Bao
DOI: 10.1016/J.CANLET.2008.09.042
关键词:
摘要: Polygonatum cyrtonema lectin (PCL), a mannose-binding lectin, has been reported to induce cytotoxicity and apoptosis. Herein, we demonstrated that PCL-induced apoptosis autophagy in A375 cells. The apoptotic mechanism was PCL treatment regulated Bax, Bcl-xL Bcl-2 proteins, leading mitochondrial depolarization, cytochrome c release caspase activation. Subsequently, found abrogated glutathione antioxidant system induced mitochondria generate ROS accumulation, resulting p38-p53 Moreover, confirmed the ROS-p38-p53 pathway involved autophagy. In conclusion, these results indicate induces via mitochondrial-mediated pathway.
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sci-hub.se 参考文章(28)
Devrim Gozuacik, Adi Kimchi, Autophagy as a Cell Death and Tumor Suppressor Mechanism Oncogene. ,vol. 23, pp. 2891- 2906 ,(2004) , 10.1038/SJ.ONC.1207521
Jie AN, Jin-Zhi LIU, Chuan-Fang WU, Jian LI, Lei DAI, Els DAMME, Jan BALZARINI, Erik CLERCQ, Fang CHEN, Jin-Ku BAO, Anti-HIV I/II activity and molecular cloning of a novel mannose/sialic acid-binding lectin from rhizome of Polygonatum cyrtonema Hua. Acta Biochimica et Biophysica Sinica. ,vol. 38, pp. 70- 78 ,(2006) , 10.1111/J.1745-7270.2006.00140.X
Ryan C. Scott, Gábor Juhász, Thomas P. Neufeld, Direct Induction of Autophagy by Atg1 Inhibits Cell Growth and Induces Apoptotic Cell Death Current Biology. ,vol. 17, pp. 1- 11 ,(2007) , 10.1016/J.CUB.2006.10.053
G Stoll, J Beuth, B Schneider, K Schumacher, T Stiefel, G Reich, J Hanisch, P R Bock, Influence of postoperative complementary treatment with lectin-standardized mistletoe extract on breast cancer patients. A controlled epidemiological multicentric retrolective cohort study. Anticancer Research. ,vol. 23, pp. 5081- 5087 ,(2003)
Elieser Gorelik, Uri Galili, Avraham Raz, On the Role of Cell Surface Carbohydrates and their Binding Proteins (lectins) in Tumor Metastasis Cancer and Metastasis Reviews. ,vol. 20, pp. 245- 277 ,(2001) , 10.1023/A:1015535427597
Michael O. Hengartner, The biochemistry of apoptosis Nature. ,vol. 407, pp. 770- 776 ,(2000) , 10.1038/35037710
Zhen Liu, Bo Liu, Zi-Ting Zhang, Ting-Ting Zhou, He-Jiao Bian, Ming-Wei Min, Yan-Hong Liu, Jing Chen, Jin-Ku Bao, A mannose-binding lectin from Sophora flavescens induces apoptosis in HeLa cells Phytomedicine. ,vol. 15, pp. 867- 875 ,(2008) , 10.1016/J.PHYMED.2008.02.025
Yan Cheng, Feng Qiu, Shin-ichi Tashiro, Satoshi Onodera, Takashi Ikejima, ERK and JNK mediate TNFα-induced p53 activation in apoptotic and autophagic L929 cell death Biochemical and Biophysical Research Communications. ,vol. 376, pp. 483- 488 ,(2008) , 10.1016/J.BBRC.2008.09.018
Michael J. May, Lisa A. Madge, Caspase Inhibition Sensitizes Inhibitor of NF-κB Kinase β-deficient Fibroblasts to Caspase-independent Cell Death via the Generation of Reactive Oxygen Species Journal of Biological Chemistry. ,vol. 282, pp. 16105- 16116 ,(2007) , 10.1074/JBC.M611115200
Myung-Sunny Kim, Jienny Lee, Kang-Min Lee, Sei-Hoon Yang, Sujinna Choi, Sang-Young Chung, Tae-Young Kim, Won-Hun Jeong, Raekil Park, Involvement of hydrogen peroxide in mistletoe lectin-II-induced apoptosis of myeloleukemic U937 cells. Life Sciences. ,vol. 73, pp. 1231- 1243 ,(2003) , 10.1016/S0024-3205(03)00418-1