TORC1 Promotes Phosphorylation of Ribosomal Protein S6 via the AGC Kinase Ypk3 in Saccharomyces cerevisiae
作者： Asier González , Mitsugu Shimobayashi , Tobias Eisenberg , David Adrian Merle , Tobias Pendl
DOI: 10.1371/JOURNAL.PONE.0120250
关键词:
摘要: The target of rapamycin complex 1 (TORC1) is an evolutionarily conserved sensor nutrient availability. Genetic and pharmacological studies in the yeast Saccharomyces cerevisiae have provided mechanistic insights on regulation TORC1 signaling response to nutrients. Using a highly specific antibody that recognizes phosphorylation bona fide ribosomal protein S6 (Rps6) yeast, we found nutrients rapidly induce Rps6 TORC1-dependent manner. Moreover, demonstrate Ypk3, AGC kinase which exhibits high homology human (S6K), required for vivo. completely abolished cells lacking Ypk3 (ypk3Δ), whereas Sch9, previously reported be ortholog S6K, dispensable phosphorylation. Phosphorylation-deficient mutations regulatory motifs abrogate phosphorylation, complementation ypk3Δ with restores rapamycin-sensitive Our findings critical component pathway use phospho-S6 offers valuable tool identify new nutrient-dependent targets
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Thomas Beck, Michael N. Hall, The TOR signalling pathway controls nuclear localization of nutrient-regulated transcription factors Nature. ,vol. 402, pp. 689- 692 ,(1999) , 10.1038/45287
C. Kruse, S. P. Johnson, J. R. Warner, Phosphorylation of the yeast equivalent of ribosomal protein S6 is not essential for growth. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 82, pp. 7515- 7519 ,(1985) , 10.1073/PNAS.82.22.7515
Antonio Casamayor, Pamela D. Torrance, Takayasu Kobayashi, Jeremy Thorner, Dario R. Alessi, Functional counterparts of mammalian protein kinases PDK1 and SGK in budding yeast Current Biology. ,vol. 9, pp. 186- 197 ,(1999) , 10.1016/S0960-9822(99)80088-8
Y. V. Budovskaya, J. S. Stephan, S. J. Deminoff, P. K. Herman, An evolutionary proteomics approach identifies substrates of the cAMP-dependent protein kinase Proceedings of the National Academy of Sciences of the United States of America. ,vol. 102, pp. 13933- 13938 ,(2005) , 10.1073/PNAS.0501046102
Paola Fabrizio, Fabiola Pozza, Scott D Pletcher, Christi M Gendron, Valter D Longo, Regulation of longevity and stress resistance by Sch9 in yeast. Science. ,vol. 292, pp. 288- 290 ,(2001) , 10.1126/SCIENCE.1059497
Laura R. Pearce, David Komander, Dario R. Alessi, The nuts and bolts of AGC protein kinases Nature Reviews Molecular Cell Biology. ,vol. 11, pp. 9- 22 ,(2010) , 10.1038/NRM2822
Robbie Loewith, Michael N. Hall, Target of Rapamycin (TOR) in Nutrient Signaling and Growth Control Genetics. ,vol. 189, pp. 1177- 1201 ,(2011) , 10.1534/GENETICS.111.133363
Teunis J. P. van Dam, Fried J. T. Zwartkruis, Johannes L. Bos, Berend Snel, Evolution of the TOR pathway. Journal of Molecular Evolution. ,vol. 73, pp. 209- 220 ,(2011) , 10.1007/S00239-011-9469-9
Alexandre Soulard, Adiel Cohen, Michael N Hall, TOR signaling in invertebrates Current Opinion in Cell Biology. ,vol. 21, pp. 825- 836 ,(2009) , 10.1016/J.CEB.2009.08.007
Claudio De Virgilio, Robbie Loewith, The TOR signalling network from yeast to man The International Journal of Biochemistry & Cell Biology. ,vol. 38, pp. 1476- 1481 ,(2006) , 10.1016/J.BIOCEL.2006.02.013