Differential transcription from the long terminal repeats of integrated avian leukosis virus DNA.

摘要: Abstract In avian leukosis virus-induced lymphoma and erythroblastosis, the expression of proto-oncogenes c-myc c-erbB is activated by downstream or readthrough transcripts initiated within integrated proviral DNA. To determine relative abundance viral RNAs extending into cellular sequences independently effects that may be exerted specific sites integration, we examined RNA infected fibroblasts. Using a nuclease protection strategy to detect downstream, readthrough, normal distinguish them from each other, found 3' long terminal repeat, i.e., transcripts, were undetectable in fibroblasts could not have amounted more than 1 2% total RNA. However, RNAs, which are 5' repeat extended beyond polyadenylation site DNA, present at relatively high levels, making up approximately 15%