A simple, efficient method for the separate isolation of RNA and DNA from the same cells.

摘要: A method for the isolation of total cytoplasmic RNA and high molecular weight DNA from same cells is described. Cells are gently lysed with NP40 in presence vanadyl ribonucleoside complex nuclei pelleted by centrifugation. purified phenol/CHCl3 extraction lysate supernatant followed ethanol precipitation. Protein removed salt-precipitation after digested proteinase K sodium dodecyl sulfate. High yields clean, intact obtained. major advantage that it can be scaled down to quantitatively extract as little 1000 cells.