LRSim: A Linked-Reads Simulator Generating Insights for Better Genome Partitioning.
作者： Ruibang Luo , Fritz J. Sedlazeck , Charlotte A. Darby , Stephen M. Kelly , Michael C. Schatz
DOI: 10.1016/J.CSBJ.2017.10.002
关键词:
摘要: Linked-read sequencing, using highly-multiplexed genome partitioning and barcoding, can span hundreds of kilobases to improve de novo assembly, haplotype phasing, other applications. Based on our analysis 14 datasets, we introduce LRSim that simulates linked-reads by emulating the library preparation sequencing process with fine control over variants, linked-read characteristics, short-read profile. We conclude from phasing assembly multiple recommendations coverage, fragment length, when genomes different sizes complexities. These optimizations results orders magnitude, enable development novel methods. is available at https://github.com/aquaskyline/LRSIM.
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