Effect of T cell subset dose on outcome of T cell-depleted bone marrow transplantation

摘要: T cell depletion using the murine monoclonal antibody (moAb) T10B9 is unique in that receptor (TCR)gamma delta bearing subset relatively spared compared to TCR alpha beta + subset. We evaluated probabilities of engraftment, acute and chronic graft-versus-host disease (GVHD), relapse, survival 273 recipients marrow depleted T10B9. Sixty-two patients received from an HLA-identical sibling, 54 partially matched related donor 157 unrelated marrow. Limiting dilution analysis (LDA) was used estimate total clonable dose all a modified LDA moAb-coated immunomagnetic beads +, CD4+, CD8+ cells patients. gamma estimated by flow cytometry. Cox proportional hazards regression models were assess impact dose/kg body weight on outcome. found significant association (P = 0.004), but not or dose, with probability engraftment. significantly associated < 0.001) risks grade 2-4 GVHD donors donors. Neither nor any be GVHD, relapse survival. The results confirm preclinical studies showing promote are increased risk while engraftment grafts These findings support hypothesis subsets differentially contribute alloengraftment GVHD.