Genetics of Bacillus subtilis chemotaxis: isolation and mapping of mutations and cloning of chemotaxis genes.

摘要: We isolated a collection of chemotaxis mutants and characterized them for chemotactic phenotype genotype. The mutations most these mapped in the region between pyrD thyA. However, mutation gene specifying methyltransferase very close to aroF. From bank phages containing Bacillus subtilis DNA we identified two lambda charon4 that contained genes functions. inserted DNAs were removed by digestion with restriction endonuclease EcoRI found share 4.0-kilobase (kb) fragment. One also 7.7-kb fragment, other 10.9-kb complemented each fragments ligated into plasmid pFH7 incorporated lysogenic SP beta c2 or deletion mutant order form transducing phages. thyA tested complementation 4.0-kb fragment plus A total 24 7 9 15 25 none fragments.